Coding

Part:BBa_K5301023

Designed by: Jiaxin Cao   Group: iGEM24_BNU-China   (2024-09-24)
Revision as of 09:36, 1 October 2024 by Guanjiaxin (Talk | contribs)

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This pathway expresses SCSdC-mCh[1-10] using T7 expression system.

This pathway expresses SCSdC-mCh[1-10] using T7 expression system.

Usage and Biology

We used this pathway to express SCSdC-mCh[1-10] by using T7 expression system in E.coli, and the expression of SCSdC-mCh[1-10] can be induced by IPTG. For more information on SCSdC-mCh[1-10], go to BBa_K5301005.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 1764
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1283
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1079
    Illegal AgeI site found at 1872
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None