Composite
Part:BBa_K5301029
Designed by: Zihan Wen Group: iGEM24_BNU-China (2024-09-25)
Revision as of 09:33, 1 October 2024 by Guanjiaxin (Talk | contribs)
spNW15 expression pathway (T7)
This pathway expresses the SpyCatcher-NW15-SpyTag fusion protein under IPTG induction, achieving self-cyclization of NW15 to construct nanodiscs.
Usage and Biology
We used this pathway to express spNW15 by using T7 expression system in E.coli, and the expression of spNW15 can be induced by IPTG. For more information on spNW15, go to BBa_K5301013.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 571
Illegal SpeI site found at 523
Illegal PstI site found at 739 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 571
Illegal NheI site found at 139
Illegal NheI site found at 1175
Illegal SpeI site found at 523
Illegal PstI site found at 739 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 571
Illegal BglII site found at 627
Illegal BglII site found at 999
Illegal BamHI site found at 172 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 571
Illegal SpeI site found at 523
Illegal PstI site found at 739 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 571
Illegal SpeI site found at 523
Illegal PstI site found at 739
Illegal AgeI site found at 1102 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |