Composite

Part:BBa_K5301028

Designed by: Zihan Wen   Group: iGEM24_BNU-China   (2024-09-25)
Revision as of 09:31, 1 October 2024 by Guanjiaxin (Talk | contribs)

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spMSP1D1 expression pathway (T7)

Usage and Biology

This pathway expresses the SpyCatcher-MSP1D1-SpyTag fusion protein (spMSP1D1) under IPTG-induced conditions, achieving self-cyclization of MSP1D1 to construct nanodiscs.For more information on spMSP1D1, go to BBa_K5301011.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 571
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 571
    Illegal NheI site found at 139
    Illegal NheI site found at 1145
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 571
    Illegal BglII site found at 999
    Illegal BamHI site found at 172
    Illegal XhoI site found at 895
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 571
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 571
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None