Composite

Part:BBa_K5301025

Designed by: Yiran Cheng   Group: iGEM24_BNU-China   (2024-09-25)
Revision as of 09:26, 1 October 2024 by Guanjiaxin (Talk | contribs)

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novel SEND system

A novel SEND system based on BBa_K4140013. VLP formed by PEG10 can carry GFP mRNA, and vesicles containing VSVg protein on the surface can assist VLP to transport between cells and infect target cells. The success of infection can be indicated by the expression of GFP.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 3244
    Illegal PstI site found at 3946
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2056
    Illegal NheI site found at 3316
    Illegal PstI site found at 3244
    Illegal PstI site found at 3946
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2439
    Illegal BamHI site found at 2676
    Illegal BamHI site found at 3359
    Illegal BamHI site found at 4594
    Illegal XhoI site found at 1836
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 3244
    Illegal PstI site found at 3946
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 3244
    Illegal PstI site found at 3946
    Illegal NgoMIV site found at 1647
    Illegal AgeI site found at 2575
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4638
    Illegal BsaI.rc site found at 4478
    Illegal SapI.rc site found at 4701


[edit]
Categories
Parameters
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