Part:BBa_K5034201

pBBR1MCS-terminator plasmid

Description

The pBBR1MCS plasmid is a synthetic plasmid backbone used in various genetic engineering applications in microbial systems. We modified the original pBBR1MCS and added a double terminator rrnBT1-T7Te(BBa_B0015) downstream the Biobrick suffix.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4981
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NotI site found at 9
  Illegal NotI site found at 2834
  Illegal NotI site found at 4987
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  Illegal EcoRI site found at 4981
  Illegal BglII site found at 3580
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal prefix found at 4981
  Illegal suffix found at 2
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal prefix found at 4981
  Plasmid lacks a suffix.
  Illegal XbaI site found at 4996
  Illegal SpeI site found at 2
  Illegal PstI site found at 16
  Illegal NgoMIV site found at 562
  Illegal NgoMIV site found at 4244
  Illegal NgoMIV site found at 4527
  Illegal AgeI site found at 402
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Plasmid lacks a prefix.
  Plasmid lacks a suffix.
  

The plasmid backbone map is shown in the figure.(Fig.1)

Figure 1: The plasmid map of pBBR1MCS-terminator

Applications

1. It can be used in microbial fuel cells to study and enhance the extracellular electron transfer capabilities such as in S. oneidensis.

2. It can be used as a backbone for CRISPR-based genome editing systems, enabling precise genetic modifications.

Reference

Yang, Y., Wu, Y., Hu, Y., Cao, Y., Poh, C. L., Cao, B., & Song, H. (2015). Engineering Electrode-Attached Microbial Consortia for High-Performance Xylose-Fed Microbial Fuel Cell. ACS Catalysis, 5(11), 6937-6945. DOI: 10.1021/acscatal.5b01733.