DNA
PEBP-GFP

Part:BBa_K5291051

Designed by: Yuning Wu   Group: iGEM24_BNUZH-China   (2024-10-01)
Revision as of 08:13, 1 October 2024 by Registry (Talk | contribs)

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PEBP-GFP

This part is design to anchor the PEBP-GFP fusion protein to the membrane of bacteria. bacteria with PE-binding peptide(PEBP) are expected to have a stronger PE plastic binding capacity. And GFP is used to observe the bacteria on the PE surface to detect the binding ability of the engineering bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 895
    Illegal PstI site found at 905
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 895
    Illegal PstI site found at 905
    Illegal NotI site found at 109
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 895
    Illegal BamHI site found at 858
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 895
    Illegal PstI site found at 905
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 895
    Illegal PstI site found at 905
    Illegal NgoMIV site found at 1213
    Illegal NgoMIV site found at 1582
    Illegal AgeI site found at 256
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None