Regulatory

Part:BBa_K239000:Experience

Designed by: Axel Nystrom   Group: iGEM09_UCL_London   (2009-06-20)
Revision as of 02:12, 22 October 2009 by Zcbcd06 (Talk | contribs) (→‎Applications of BBa_K239000)

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Applications of BBa_K239000

We hope that it also can be used to detect presence of relevant unfolded proteins in the periplasmic space (e.g. target pharmaceuticals), to detect shear stress or other so far undefined relevant stress.

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The DegP promoter is dependent of Sigma E (and hence also the growth phase) and can not be induced only by over expression of e.g. CpxR. In comparison, the related Spy promoter (BBa_K239001), which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Experiments by the The UCL_London_2009 team have also showed that the activity of the DegP promoter, compared to the spy promoter, is much more dependent of the growth phase of the bacteria.

For detailed information please visit [http://2009.igem.org/Team:UCL_London/From_the_lab/Expts UCL_London Wet Lab Experiments] & [http://2009.igem.org/Team:UCL_London/From_the_lab/Results UCL_London Wet Lab Results]