Composite

Part:BBa_K5166040

Designed by: Sihui Liu; Yancheng Zeng; Xiao Han; Zhirong Wang   Group: iGEM24_BIT-China   (2024-10-01)
Revision as of 07:55, 1 October 2024 by Registry (Talk | contribs)

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pGAPZα-mntR-pir

The BIT-China team constructed a yeast surface display that could link MntR to anchor proteins to be displayed on the surface of Pichia pastoris.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1677
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1174
    Illegal NotI site found at 1663
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 730
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1677
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1677
    Illegal AgeI site found at 1783
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 238


[edit]
Categories
//chassis/eukaryote/pichia
Parameters
None