Coding
BcsB

Part:BBa_K5291009:Design

Designed by: Wendan Zheng   Group: iGEM24_BNUZH-China   (2024-08-28)
Revision as of 07:24, 1 October 2024 by Kortybones (Talk | contribs) (→‎References)

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BcsB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 805
    Illegal PstI site found at 838
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 805
    Illegal PstI site found at 838
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 805
    Illegal PstI site found at 838
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 805
    Illegal PstI site found at 838
    Illegal NgoMIV site found at 729
    Illegal NgoMIV site found at 1359
    Illegal AgeI site found at 1439
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design considerations for the BcsB genes in bacterial cellulose biosynthesis involve their essential roles in forming the cellulose synthase complex, ensuring proper membrane integration, and facilitating efficient cellulose chain synthesis and export.


Source

Pseudomonas putida NBRC 14164

References

Römling, U. & Galperin, M.Y. Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions. Trends in Microbiology 23, 545-557 (2015).