Coding
BcsA

Part:BBa_K5291008:Design

Designed by: Wendan Zheng   Group: iGEM24_BNUZH-China   (2024-08-28)
Revision as of 07:23, 1 October 2024 by Kortybones (Talk | contribs) (→‎References)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


BcsA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2263
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2263
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2263
    Illegal BglII site found at 1508
    Illegal BamHI site found at 1185
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2263
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2263
    Illegal NgoMIV site found at 246
    Illegal NgoMIV site found at 783
    Illegal NgoMIV site found at 795
    Illegal NgoMIV site found at 1021
    Illegal AgeI site found at 1975
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The design considerations for the BcsA genes in bacterial cellulose biosynthesis involve their essential roles in forming the cellulose synthase complex, ensuring proper membrane integration, and facilitating efficient cellulose chain synthesis and export.


Source

Pseudomonas putida NBRC 14164

References

Römling, U. & Galperin, M.Y. Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions. Trends in Microbiology 23, 545-557 (2015).