Plasmid

Part:BBa_K5302014:Design

Designed by: Bojun Yang   Group: iGEM24_USTC   (2024-10-01)
Revision as of 06:37, 1 October 2024 by Registry (Talk | contribs)

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pBBR-intimin-mCherry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4483
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 4804
    Illegal PstI site found at 2016
    Illegal PstI site found at 3494
    Illegal PstI site found at 3742
    Illegal PstI site found at 4189
    Illegal PstI site found at 4792
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4483
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 4804
    Illegal PstI site found at 2016
    Illegal PstI site found at 3494
    Illegal PstI site found at 3742
    Illegal PstI site found at 4189
    Illegal PstI site found at 4792
    Illegal NotI site found at 1057
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4483
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 4804
    Illegal BglII site found at 1803
    Illegal XhoI site found at 5149
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4483
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 4804
    Illegal PstI site found at 2016
    Illegal PstI site found at 3494
    Illegal PstI site found at 3742
    Illegal PstI site found at 4189
    Illegal PstI site found at 4792
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4483
    Illegal EcoRI site found at 4531
    Illegal EcoRI site found at 4804
    Illegal PstI site found at 2016
    Illegal PstI site found at 3494
    Illegal PstI site found at 3742
    Illegal PstI site found at 4189
    Illegal PstI site found at 4792
    Illegal NgoMIV site found at 2467
    Illegal NgoMIV site found at 2750
    Illegal NgoMIV site found at 5740
    Illegal AgeI site found at 5010
    Illegal AgeI site found at 5580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1379
    Illegal SapI.rc site found at 2316
    Illegal SapI.rc site found at 2526


Design Notes

Please be advised that all listed genes have undergone codon optimization for our chassis strain, Escherichia coli Nissle 1917 (EcN). For those considering alternative chassis organisms, it is recommended to reassess the necessity of codon optimization.


Source

This work is derived from pBBR1MCS-2, pUC19-intimin-spytag and pET-21b(+)-mCherry, and it has undergone codon optimization.

References