Device

Part:BBa_K239009:Experience

Designed by: Xiang Chen   Group: iGEM09_UCL_London   (2009-09-06)
Revision as of 01:52, 22 October 2009 by Zcbcd06 (Talk | contribs) (→‎User Reviews)

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Applications of BBa_K239009

This part was designed to test promoter spy, BBa_K239001. It can be used to detect misfolding of proteins in the periplasm or shear stress in bio-processing.

User Reviews

UNIQda42252d98eee06b-partinfo-00000000-QINU UNIQda42252d98eee06b-partinfo-00000001-QINU

The device responded to various periplasmic stresses such as Copper (4mM CuCl2), Indole (4mM) and Ethanol (5%). Possibly it can also be used to detect presence of heterogeneous unfolded proteins in the periplasmic space (e.g. target pharmaceuticals) or to detect high levels of shear stress during cultivation in a bioreactor.

Compare to the related DegP promoter (BBa_K239000), Spy promoter, which is dependent on sigma factor 70, can be induced up to 40 fold by over expression of CpxR. (Bury-Moné et al. 2009) Most importantly, spy promoter showed no respond to stationary phase.

Detailed information please check [http://2009.igem.org/Team:UCL_London/From_the_lab/Results#Growth_Cruves_Experiments_II UCL_London iGEM Team Wiki Growth_Cruves_Experiments_II]