Coding

Part:BBa_K227005:Design

Designed by: WashU iGEM 2009   Group: iGEM09_Wash_U   (2009-10-12)
Revision as of 01:52, 22 October 2009 by Jathman (Talk | contribs) (→‎Source)

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puc B coding region from R. sphaeroides


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

puc A and puc B together create the antennae complex necessary to harvest varying intensities of light. A functional LHII complex requires both puc A and B. Data suggests that A common problem in bioreactors is the uneven distribution of light between cells nearest the light source and cells farther away from the light source. One way of combating this problem is modify the antenna size of the organism. By truncating the light-harvesting antenna, cells at the exterior of the bioreactor waste less light through Non-Photochemical Quenching, thus allowing photons to penetrate deeper and reach cells in the interior of the bioreactor. This leads to an increase in photosynthetic efficiency and productivity for the entire culture.

This pucB gene contains the natural R. sphaeroides RBS.

Source

PCR applified from plasmid pRKCBC3 provided by Dr. C. Neil Hunter, University of Sheffield, Molecular Biology and Biotechnology.

References

Kiley, Patricia J.; and Kaplan, Samuel; "Cloning, DNA , and Expression of the Rhodobacter sphaeroides Light-Harvesting B800-850-a and B800-850-b Genes" Journal of Bacteriology. July 1987.[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC212379/pdf/jbacter00197-0368.pdf]