Coding

Part:BBa_K5382124

Designed by: yuyanyan chen   Group: iGEM24_HUBU-4-CHN   (2024-09-25)
Revision as of 06:05, 1 October 2024 by Chenyuyanyan (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


CL7-protein tag

CL7 is a protein tag engineered from colicin CE7. In E.coli, CE7 is a bacteriocin that belongs to the colicin DNase family and has endonuclease activity. Through engineering, CL7 removed DNA binding and catalytic activity, but retained its high affinity binding ability to the corresponding inhibitory protein Im7. This property makes CL7 a useful tool for protein purification and molecular biology applications.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 133
    Illegal BglII site found at 81
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 133
    Illegal AgeI site found at 31
    Illegal AgeI site found at 45
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None