Coding
nqrf

Part:BBa_K5291004:Design

Designed by: Wendan Zheng   Group: iGEM24_BNUZH-China   (2024-08-28)
Revision as of 04:08, 1 October 2024 by Kortybones (Talk | contribs) (→‎Source)


NqrF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 976
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 976
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 976
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 976
    Illegal NgoMIV site found at 150
    Illegal NgoMIV site found at 154
    Illegal NgoMIV site found at 493
    Illegal NgoMIV site found at 847
    Illegal NgoMIV site found at 890
    Illegal AgeI site found at 1054
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The NqrF subunit is a crucial component of the Nqr complex, which serves as a type II NADH dehydrogenase in the electron transport chain (ETC) of the bacterium Pseudomonas aeruginosa. NqrF, along with other subunits in the Nqr complex, plays a significant role in the transfer of electrons from NADH to ubiquinone, a process essential for cellular respiration and energy conservation. The Nqr complex is particularly important because it has been identified as the primary phenazine reductase in P. aeruginosa, capable of reducing phenazine compounds, which are redox-active secondary metabolites with various physiological functions, including supporting energy conservation and antibiotic tolerance.The NqrF subunit and the Nqr complex can be harnessed to improve the efficiency of biocatalytic processes, especially under anaerobic conditions where phenazine compounds act as electron shuttles. Researchers can utilize the Nqr complex, and specifically the NqrF subunit, to engineer microbial strains with enhanced capabilities for extracellular electron transfer, a process that can be leveraged in the production of biofuels, bioremediation, and the synthesis of value-added chemicals.To use NqrF in a project, one would typically clone the gene encoding this subunit into an expression vector and transform it into a suitable host organism, such as Escherichia coli or P. aeruginosa itself. Once expressed, the NqrF subunit can be purified for further study or incorporated into a metabolic pathway engineered for a specific application.It should be noted that the functional verification can be completed by the change of the content of biological NADH in the chassis after the use of this element.

Source

The NqrF subunit is derived from the Pseudomonas aeruginosa.

References