Coding

Part:BBa_K5302004

Designed by: Dekun Zhou   Group: iGEM24_USTC   (2024-10-01)
Revision as of 04:01, 1 October 2024 by Registry (Talk | contribs)

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Z3C

Just like miniZ, Z3C is also derived from three helix 58-residue Z-domain of staphylococcal protein A, but it has three helix. Z3C also shows great affinity with VEGF(KD=41 nM). We used pBBR plasmid as a backbone and transfered Z3C into Escherichia coli Nissle 1917, and finally succeeded in expressing Z3C.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//cds/receptor
Parameters
biologyEscherichia coli Nissle 1917