Part:BBa_K5097011

Acid-sensor, Base-producer pASR-PUT1

Usage and Biology

This composite part functions as an acid sensing, base producing part. It contains the coding sequences speA and speB, the two enzymes involved in the conversion of arginine to putrescine (Morris., et al 1969). These genes, when expressed, will produce putrescine, a base. Expression of these genes is controlled by pASR, an acid sensitive promoter sequence from the parts repository. The 2024 Oneonta iGEM team designed this sequence for use in pH adjusting devices for use in the remediation of wastewater from the manufacturing of microprocessors.

Figure 1: Conversion of Arginine to Putrescine. Figure adapted from reference 2.

References

1-Morris, D and Koffron, K. “Putrescine Biosynthesis in Escherichia coli: REGULATION THROUGH PATHWAY SELECTION” Journal of biological chemistry, vol. 244, 22, (1969): 6094-9. doi.org/10.1016/S0021-9258(18)63510-0.

2-Charlier, Daniel & Bervoets, Indra. (2019). Regulation of arginine biosynthesis, catabolism and transport in Escherichia coli. Amino Acids. 51. 10.1007/s00726-019-02757-8.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 2930
  Illegal BamHI site found at 1541
  Illegal BamHI site found at 2843
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 1319
  Illegal AgeI site found at 2865
  
• 1000
  COMPATIBLE WITH RFC[1000]