Composite
Part:BBa_K5236022
Designed by: Hening Guo Group: iGEM24_BASIS-China (2024-10-01)
pET28a-pelB-BhrPETase(M57L)-6xHis
T7 promoter: used to regulate gene expression of recombinant proteins. Lac operator: used to control gene expression by lactose. RBS: the location to bind ribosome. BhrPETase M57L: This sequence encodes for a mutated BhrPETase M57L and is derived from Escherichia coli. pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane. 6xHis: a tag for the purification of protein which focus in the production of recombinant proteins. T7 terminator: terminates transcription.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 308
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 218
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 912
[edit]
Categories
Parameters
None |