Composite

Part:BBa_K5102086:Design

Designed by: Natalia Kuźmierkiewicz   Group: iGEM24_Munich   (2024-10-01)
Revision as of 02:39, 1 October 2024 by Nataliak-2024 (Talk | contribs) (→‎Design Notes)

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pU6_gRNA1-ProgRAM_tape1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

gRNA 1 encoded under the pU6 promoter with a 30 bp spacer, guiding PspCas13b-ADAR2DD complex to the first AUG in the ProgRAM_tape1, and the PspCas13b stem loop.

Source

Part assembled from the backbone available in lab and oligos from a DNA synthesis provider.

References