Composite

Part:BBa_K5236015

Designed by: Hening Guo   Group: iGEM24_BASIS-China   (2024-10-01)
Revision as of 02:31, 1 October 2024 by Registry (Talk | contribs)

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pET28a-pelB-IsPETase(K95S)-6xHis

T7 promoter: used to regulate gene expression of recombinantproteins. Lac operator: used to control gene expression by lactose. RBS: the location to bind ribosome. IsPETase K95S: This sequence encodes for a mutated IsPETase K95S and is derived from Escherichia coli. pelB: the sequence codes for signal peptide which helps PETase exit the cell membrane. 6xHis: a tag for the purification of protein which focus in the production of recombinant proteins. T7 terminator: terminates transcription.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1005


[edit]
Categories
Parameters
None