Plasmid
pEX

Part:BBa_K243033:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-20)
Revision as of 01:13, 22 October 2009 by Sarah (Talk | contribs) (→‎Design Notes)

pEX


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 2734
    Illegal XbaI site found at 1523
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 2735
    Illegal PstI site found at 2749
    Illegal NotI site found at 2742
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1808
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 2735
    Illegal XbaI site found at 1523
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal suffix found in sequence at 2725
    Illegal XbaI site found at 1523
    Illegal NgoMIV site found at 1531
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3800
    Illegal SapI.rc site found at 4799


Design Notes

Commented GenBank file

Source

By adding additional restriction sites in pMAL we create our expression vector.

References