Primer

Part:BBa_K5079014

Designed by: Marianna Florez   Group: iGEM24_Queens-Canada   (2024-09-28)
Revision as of 01:51, 1 October 2024 by Mariannaflorez (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


K45_CP_P95 Switch primers into pET-28a Switch 7(Rev)

This part is the reverse primer for switch 7 (K45_CP_P95, part BBa_K5079032) into pET-28a. It allows for the addition of overhangs to the sequence of protein switch 7 that are complementary to the pET-28a sequence for cloning. It has a melting point of 67.0°C . Part "BBa_ K5079013" used as forward primer. Cloned in frame with a His Tag. Used with q5 polymerase mastermix.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 5
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None