Coding

Part:BBa_K5237003

Designed by: Marik Mueller   Group: iGEM24_Heidelberg   (2024-10-01)
Revision as of 01:28, 1 October 2024 by Registry (Talk | contribs)

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Cas Staple: SV40 NLS-dMbCas12a-dSpCas9-Nucleoplasmin NLS



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 8026
    Illegal PstI site found at 325
    Illegal PstI site found at 346
    Illegal PstI site found at 658
    Illegal PstI site found at 1813
    Illegal PstI site found at 3100
    Illegal PstI site found at 3375
    Illegal PstI site found at 4663
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 8026
    Illegal PstI site found at 325
    Illegal PstI site found at 346
    Illegal PstI site found at 658
    Illegal PstI site found at 1813
    Illegal PstI site found at 3100
    Illegal PstI site found at 3375
    Illegal PstI site found at 4663
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 8026
    Illegal BglII site found at 2276
    Illegal BglII site found at 2402
    Illegal BglII site found at 2909
    Illegal BglII site found at 2955
    Illegal BglII site found at 3002
    Illegal BglII site found at 3278
    Illegal BglII site found at 3362
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 8026
    Illegal PstI site found at 325
    Illegal PstI site found at 346
    Illegal PstI site found at 658
    Illegal PstI site found at 1813
    Illegal PstI site found at 3100
    Illegal PstI site found at 3375
    Illegal PstI site found at 4663
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 8026
    Illegal PstI site found at 325
    Illegal PstI site found at 346
    Illegal PstI site found at 658
    Illegal PstI site found at 1813
    Illegal PstI site found at 3100
    Illegal PstI site found at 3375
    Illegal PstI site found at 4663
    Illegal NgoMIV site found at 406
    Illegal NgoMIV site found at 838
    Illegal NgoMIV site found at 1405
    Illegal NgoMIV site found at 1483
    Illegal NgoMIV site found at 2455
    Illegal NgoMIV site found at 3356
    Illegal NgoMIV site found at 4951
  • 1000
    COMPATIBLE WITH RFC[1000]


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