Part:BBa_K5439006

FRET-based system for the detection of ibuprofen

FRET-based sensor system for the detection of cadmium and other heavy metals that consists of long-chain fatty acid CoA ligase from Sphingomonas spp.(BBa_K5439005),an enzyme that catalyzes the conversion of ibuprofen into isobutylcatechol, flanked by two fluorescent proteins: ECFP(BBa_K1159302)as energy donor and mVenus(BBa_K1907000)as an energy acceptor.

Usage and Biology

Ibuprofen is an anti-inflammatory treatment drug widely used in the world that can be bought without any necessary prescription. This makes ibuprofen a drug that everyone can consume easily, bringing problems because its disposal makes it an emerging contaminant in water bodies Jan-Roblero, J., & Cruz-Maya, J. A.(2023). Due to this emerging contaminant, new ways to find effective treatments that can remove ibuprofen from water are needed; one particular approach has been studied which involves using different organisms that can degrade ibuprofen by metabolic pathways. An example of it is Sphingomonas Ibu-2; an organism that has been grown in an environment rich in ibuprofen. The described organism has the ability to metabolize ibuprofen to isobutylcatechol due to the adaptation, which one particular gene is in charge of this degradation which is IpfF . The enzyme chosen for the generation of the biopart was CoA ligase (EC:6.2.1.3) as a detector for the presence of ibuprofen. The enzyme catalyzes the conversion of ibuprofen into isobutylcatechol Murdoch, R. W. (2013).

[1]. Jan-Roblero, J., & Cruz-Maya, J. A. (2023). Ibuprofen: Toxicology and Biodegradation of an Emerging Contaminant. Molecules (Basel, Switzerland), 28(5), 2097. https://doi.org/10.3390/molecules28052097

[2]. Murdoch, R. W., & Hay, A. G. (2013). Genetic and chemical characterization of ibuprofen degradation by Sphingomonas Ibu-2. Microbiology (Reading, England), 159(Pt 3), 621–632. https://doi.org/10.1099/mic.0.062273-0