Composite

Part:BBa_K5047039:Design

Designed by: Clément Alain Serge Corneil Vanmerris   Group: iGEM24_UNILausanne   (2024-09-30)
Revision as of 21:41, 30 September 2024 by Registry (Talk | contribs)

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This sequence enables constitutive Argonaute expression in yeast.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5283
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 5191
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3711
    Illegal AgeI site found at 5338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5860


Design Notes

None


Source

The promoter comes from the pVW222 plasmid given to us by Prof. Serge Pelet (DMF, university of Lausanne). The protein coding sequence and the terminator were synthetically designed to ensure compatibility and optimal function in yeast.

References