Composite

Part:BBa_K5508001:Design

Designed by: Yaoyao Liang   Group: iGEM24_Hangzhou-MedX   (2024-09-30)
Revision as of 20:19, 30 September 2024 by Registry (Talk | contribs)

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GAL1,10-HsLF-CYC1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 960
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 960
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1375
    Illegal BglII site found at 1499
    Illegal BglII site found at 2486
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 960
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 960
    Illegal NgoMIV site found at 2342
    Illegal AgeI site found at 374
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1839


Design Notes

The BBa_K5508001(HsLF gene) was placed under the control of BBa_K5477005(bidirectional promoters Gal1,10), and the BBa_K3384311(CYC1 terminator) was responsible for terminating its transcription for efficient expression of the lactoferrin gene.


Source

None

References