Composite

Part:BBa_K5206008

Designed by: Yaoyao Liang   Group: iGEM24_Nanjing-BioX   (2024-09-30)
Revision as of 19:53, 30 September 2024 by Registry (Talk | contribs)

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PrT7-ctrpiB

The BBa_K5206007 (ribose-5-phosphate isomerase, RpiB) derived from Clostridium thermocellum was ligated to pET-28a(+) to construct the recombinant plasmid. The BBa_I719005(T7 promoter) and BBa_K731721(T7 terminator) respectively control the start and termination of transcription of this gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 487
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 228
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None