Part:BBa_K5150001

Bioactive multipeptide optimized for E.coli

Multipeptide sequence 1

This multipeptide protein was carefully designed to integrate peptides with antidiabetic, antihypertensive, hypocholesterolemic and antithrombotic properties from food sources such as quinoa, soybean, goat milk, cow milk and calpis. It is optimized to be highly expressed in E. coli enabling straightforward purification through affinity chromatography.

The main objective is to encapsulate the protein for oral ingestion so it could be released in the gut. Once released, the bioactive peptides are absorbed into the bloodstream, where they can modulate key metabolic pathways linked to diabetes and its complications. The protein can be used in projects related to nutrition studies, therapeutic natural products, food development or investigation of digestive proteases.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal XhoI site found at 400
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 150
  
• 1000
  COMPATIBLE WITH RFC[1000]