Composite
PBad-clyA-

Part:BBa_K257011:Experience

Designed by: Soufiane Boumahdi   Group: iGEM09_Paris   (2009-10-17)
Revision as of 00:31, 22 October 2009 by Sboumahdi (Talk | contribs) (→‎Applications of BBa_K257011)

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Applications of BBa_K257011

Pbad-clyA-RFP on arabinose medium

TolA clyA Ara 01.jpg


Pbad-clyA-RFP on LB medium (without arabinose)

TolA clyA LB 01.jpg

Pfec-RFP on medium with citrate (without clyA, fluorescence isn't localize on the membrane exclusively)


Citrate30.jpg

On the first image, we observed fluorescence localized to the membrane when growing bacteria transformed with PBad-ClyA-RFP on a medium with arabinose. We can compare it to 2 others images from microcoscopy : with Pbad-clyA-RFP without arabinose, the fluorescent is less intense (pBad isn't repressed so there is little expression of clyA-RFP). The third image demonstrate that without clyA, RFP isn't localize exclusively in the membrane (here, it's an example with Bba_K257023)

User Reviews

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