Composite

Part:BBa_K243015:Design

Designed by: Freiburg Bioware09   Group: iGEM09_Freiburg_bioware   (2009-10-14)
Revision as of 00:27, 22 October 2009 by Sarah (Talk | contribs) (→‎Design Notes)

Strep-DigA-Split Linker-Fok_i


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 278
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The choice of the linker, lipocalin tag and purification tag allows us several different combinations. Commented GenBank file

Source

Combined the parts by serial cloning steps.

References