Coding

Part:BBa_K5097014:Experience

Designed by: Christopher Dorce   Group: iGEM24_Oneonta   (2024-09-25)
Revision as of 18:01, 30 September 2024 by Dorcc343 (Talk | contribs)

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Applications of BBa_K5097014

Team Oneonta 2024 used this part to purify sBFP for studies on the effect of pH on the emission of this fluorescent protein. We expressed composite parts BBa_K5097016 and BBa_K5097017 in E. coli DH5alpha cells, and lysed the cells by treating with lysozyme and sonication. Proteins were purified via Ni-IMAC chromatography and eluted in 50mM sodium phosphate, 300mM sodium chloride, and 500mM imidazole at pH8.0. Proteins were concentrated and exchanged into PBS via centrifugal diafiltration.

bba-k509013-figure1.jpg
Figure 1: Coomasie stained PAGE gel showing purification of BFP-6XHis and RFP-6XHis

User Reviews

UNIQ2a16db718364d652-partinfo-00000000-QINU UNIQ2a16db718364d652-partinfo-00000001-QINU