Part:BBa_K5136226
I0500-B0034-inpnc-linker-mt3-linker-mt2a-B0015
Biology
INPNC
Ice nucleoprotein (INP), an outer membrane protein from Pseudomonas syringae, has been used as a surface anchor in many researches. The truncated version of INP, namely INPNC which contains only the N- and C-terminal portion of INP, has excellent capacity to anchor target proteins on the cell membrane (1).
MT3 and MT2A
The metallothioneins (MTs) are a class of low molecular weight and cysteine-rich metal binding proteins, and each one of them can bind to 6-9 heavy metal ions. The MTs are expressed as intracellular protein and are primarily responsible for metal regulation in cells of living organisms. General MTs can widely non-covalently bind divalent heavy metal ions, such as Zn2+,N2+, Pb2+, Hg2+, Cd2+, as well as As3+, but their effectiveness in treating Cr2O72- is not satisfactory. MT2a and MT3 are metallothioneins(MTs) found in Homo sapiens. MT2A not only has efficient adsorption capacity for ordinary metal ions, but also exhibits efficient processing capacity for Cr2O72-. And MT3 has a better adsorption effect on ordinary metal ions. (2). Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1205
Illegal NotI site found at 1717 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
Illegal BamHI site found at 1566
Illegal BamHI site found at 2211
Illegal BamHI site found at 2466 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1308
Illegal NgoMIV site found at 1641
Illegal AgeI site found at 979
Illegal AgeI site found at 1665 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
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