Composite

Part:BBa_K5136226

Designed by: Xiaoxiao Zhang   Group: iGEM24_XMU-China   (2024-09-14)
Revision as of 16:45, 30 September 2024 by Ssssff (Talk | contribs)


I0500-B0034-inpnc-linker-mt3-linker-mt2a-B0015

Biology

INPNC


Ice nucleoprotein (INP), an outer membrane protein from Pseudomonas syringae, has been used as a surface anchor in many researches. The truncated version of INP, namely INPNC which contains only the N- and C-terminal portion of INP, has excellent capacity to anchor target proteins on the cell membrane (1).

MT3 and MT2A


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NotI site found at 1717
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1566
    Illegal BamHI site found at 2211
    Illegal BamHI site found at 2466
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1308
    Illegal NgoMIV site found at 1641
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1665
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None