DNA

Part:BBa_K5237019

Designed by: Marik Mueller   Group: iGEM24_Heidelberg   (2024-09-30)
Revision as of 16:06, 30 September 2024 by Registry (Talk | contribs)

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TetR Binding Cassette

Binding casette containing 1x tetO site for TetR binding and Cas9 PAM (GGT) upstream for CRISPR/Cas9 binding. The casette can be expanded through digestion and ligation. It was used to establish the FRET assay with tetR-Oct1 Simple staple, and simulated enhancer hijacking with fgRNA and fusion dMbCas12a-dSpCas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 48
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None