Composite

Part:BBa_K5299200:Design

Designed by: Maria - Asimina Kakadiari   Group: iGEM24_Thessaly   (2024-09-23)
Revision as of 15:24, 30 September 2024 by Mkakadiari (Talk | contribs) (→‎Design Notes)

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P3.1 - BBa_B0030 - BBa_I746916 - BBa_B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 259


Design Notes

Created using the Golden Braid method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level α constructs.

Source

Promoter is BBa_K4583008, originally from [1].
RBS is BBa_B0030.
sfGFP is BBa_I746916.
Terminator is BBa_B0015.

References

  • [1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491