Coding

Part:BBa_K5097010

Designed by: Elber Lopez Hernandez, Kelly Acen, Runyararo Chaora   Group: iGEM24_Oneonta   (2024-09-30)
Revision as of 14:43, 30 September 2024 by Elberlopezh (Talk | contribs)


SpeB (Agmatinase)

Usage and Biology

This part codes for the SpeB gene from E.coli. The gene codes for agmatine ureohydrolase. The enzyme is responsible for the catabolism of agmatine. It converts agmatine into urea and putrescine, and is the second step in the metabolic pathway that converts arginine into putrescine (Morris., et 1969). The 2024 Oneonta iGEM team designed the sequence for use in a base producing circuit. When co-expressed with SpeA (BBa_K5097010) it will construct the pathway from turning arginine into putrescine. Acidic conditions will be neutralized with the production of putrescine.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 789
    Illegal BamHI site found at 702
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 724
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None