Coding
Part:BBa_K5097010
Designed by: Elber Lopez Hernandez, Kelly Acen, Runyararo Chaora Group: iGEM24_Oneonta (2024-09-30)
Revision as of 14:43, 30 September 2024 by Elberlopezh (Talk | contribs)
SpeB (Agmatinase)
Usage and Biology
This part codes for the SpeB gene from E.coli. The gene codes for agmatine ureohydrolase. The enzyme is responsible for the catabolism of agmatine. It converts agmatine into urea and putrescine, and is the second step in the metabolic pathway that converts arginine into putrescine (Morris., et 1969). The 2024 Oneonta iGEM team designed the sequence for use in a base producing circuit. When co-expressed with SpeA (BBa_K5097010) it will construct the pathway from turning arginine into putrescine. Acidic conditions will be neutralized with the production of putrescine.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 789
Illegal BamHI site found at 702 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 724
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
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