Part:BBa_K5348002
B0034-mutant 2
RBS mutants with a 108-fold reduction in intensity over B0034.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
B0034-mutant 2 (RBS2) - BBa_K5348002
Profile
| Name | B0034-mutant 2 (RBS2) |
|---|---|
| Base Pairs | 12 bp |
| Sequence | GCTCCATCCCCG |
| Origin | Synthesized |
Properties
The ribosome binding site (RBS) is a sequence located approximately 8-13 nucleotides upstream of the initiating AUG on mRNA, which can be precisely recognized by 16s rRNA through base complementarity [1]. This sequence promotes ribosome binding to mRNA, facilitating translation initiation. The length and base composition of the RBS sequence influence ribosome binding and translation efficiency to some extent [1]. A stronger RBS makes it easier for ribosomes to bind, thereby increasing gene expression levels, while a weaker RBS can reduce gene expression levels or only express under specific conditions.
Therefore, by replacing the RBS sequences that regulate the translation of the target protein, we can control the expression level of the target protein. We obtained a mutant of the original RBS (BBa_B0034) from the literature [2]. The RBS calculator showed a 108-fold decrease in the intensity of this mutant.
References
[1] Kozak, M. Initiation of translation in prokaryotes and eukaryotes. Gene, 1999, 234(2), 187-208.
[2] Ji W, Shi H, Zhang H, Sun R, Xi J, Wen D, Feng J, Chen Y, Qin X, Ma Y, Luo W, Deng L, Lin H, Yu R, Ouyang Q. A formalized design process for bacterial consortia that perform logic computing. PLoS One. 2013;8(2): e57482.
| None |