DNA

Part:BBa_K5300044

Designed by: Fan Yaxu   Group: iGEM24_CAU-China   (2024-09-30)
Revision as of 14:18, 30 September 2024 by Registry (Talk | contribs)

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PnuoA-Cas12k-double Terminator-PglnK-sgRNA-GFP-tracRNA-PnifH-gfp

To verify the function of the regulatory module, we attempted to construct a plasmid with PnuoA-Cas12k-Double terminator added in front of the regulatory module.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4445
    Illegal AgeI site found at 5
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3850


[edit]
Categories
Parameters
None