Part:BBa_K5322002:Design
Constitutive Mfp5 Expression System
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 300 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 137
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
To ensure successful expression of the animal-derived mussel foot protein Mfp5 in BL21(DE3), we conducted codon optimization of the Mfp5 sequence.
Source
The plasmid pET29a-J23119-RBS-Mfp5 utilizes the pET29a vector, commonly employed for robust gene expression in Escherichia coli. The components used in this design, including the J23119 promoter and Mfp5, are derived from the iGEM Registry of Standard Biological Parts (BBa_J23119, BBa_K1583002), with the mussel foot protein Mfp5 sourced from natural marine mussels. These parts were selected for their validated functionality and compatibility in synthetic biology applications, facilitating controlled expression within bacterial cells under specific conditions.