Part:BBa_K5335003
MS2 coat protein virus-like particle loading system
The part is composed of BBa_K5335000, BBa_K5335001, BBa_K5335002, promoter J23110, J23119, rrnB T1 terminator, and ribosome binding site B0034. The whole circuit can play the role of VLP assembly, surface display of functional proteins, and the inner inclusion of functional RNA.
Usage and Biology
Our project "Bacillus Gemini" aims to improve plant immunity while killing nematodes. After a large number of previous literature review, we chose Virus-like particles (VLPs) composed of coat proteins of bacteriophage MS2 of Escherichia coli as the platform for executive functions, and displayed insecticidal proteins and plant immune activation proteins on its surface to complete the role of killing nematodes that damage plants and activating plant immunity. At the same time, we also included a dual tandem 19bp stem-loop sequence that can be specifically bound by the MS2 coat protein to verify its loading capacity as a future RNA delivery vector. We plan to make MS2 coat protein express in the engineered bacteria and form VLP, bind to the functional protein with SpyCatcher on the surface, and enclose the RNA containing a 19-bp sequence of stem rings to form a multifunctional carrier (Figure 1.).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2317
Illegal NheI site found at 2340 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 392
Illegal BsaI site found at 821
Illegal BsaI site found at 1389
Illegal BsaI.rc site found at 2096
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