Plasmid_Backbone

Part:BBa_K5293014

Designed by: Arnaud Boudigou   Group: iGEM24_uOttawa   (2024-09-23)
Revision as of 13:22, 30 September 2024 by Arnaud-boudigou (Talk | contribs)


pHREAC_eGFP_ER

pHREAC plasmid backbone for Endoplasmic Reticulum targeted protein expression. Sap I sites are used to clone you insert (GGC & AAA are the scars) AAA Scar is part of the KDEL localisation tag. This empty plasmid backbone contains an insert not displayed on the sequence for easier cloning with mCherry (BBa_K5293020) as a screening tool

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2334
    Illegal EcoRI site found at 4032
    Illegal XbaI site found at 930
    Illegal SpeI site found at 5878
    Illegal PstI site found at 8535
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2334
    Illegal EcoRI site found at 4032
    Illegal NheI site found at 3243
    Illegal SpeI site found at 5878
    Illegal PstI site found at 8535
    Illegal NotI site found at 5732
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2334
    Illegal EcoRI site found at 4032
    Illegal BglII site found at 1297
    Illegal BglII site found at 2560
    Illegal BglII site found at 2580
    Illegal BglII site found at 9350
    Illegal BamHI site found at 936
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2334
    Illegal EcoRI site found at 4032
    Illegal XbaI site found at 930
    Illegal SpeI site found at 5878
    Illegal PstI site found at 8535
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2334
    Illegal EcoRI site found at 4032
    Illegal XbaI site found at 930
    Illegal SpeI site found at 5878
    Illegal PstI site found at 8535
    Illegal NgoMIV site found at 3044
    Illegal NgoMIV site found at 4202
    Illegal NgoMIV site found at 4728
    Illegal NgoMIV site found at 5604
    Illegal NgoMIV site found at 5728
    Illegal NgoMIV site found at 6755
    Illegal NgoMIV site found at 7346
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
//plasmidbackbone/assembly/typeiis
//plasmidbackbone/expression/constitutive
//plasmidbackbone/synthesis
Parameters
None