Composite

Part:BBa_K5207020

Designed by: Ruyi Shi   Group: iGEM24_Shanghai-BioX   (2024-09-30)
Revision as of 13:00, 30 September 2024 by Registry (Talk | contribs)

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TS-HydroxySugiol

This composite part consists of six expression cassettes, expressing SmGGPPS, SmCPS1, SmKSL, SmCPR, CYP76AH1, and CYP76AH3.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 4456
    Illegal NheI site found at 11899
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 685
    Illegal BglII site found at 3217
    Illegal BglII site found at 6298
    Illegal BglII site found at 9089
    Illegal BamHI site found at 2815
    Illegal XhoI site found at 2951
    Illegal XhoI site found at 2975
    Illegal XhoI site found at 4268
    Illegal XhoI site found at 4478
    Illegal XhoI site found at 4950
    Illegal XhoI site found at 8131
    Illegal XhoI site found at 11232
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2620
    Illegal NgoMIV site found at 4037
    Illegal NgoMIV site found at 4601
    Illegal NgoMIV site found at 8578
    Illegal NgoMIV site found at 13298
    Illegal AgeI site found at 861
    Illegal AgeI site found at 11195
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5196
    Illegal BsaI site found at 8117
    Illegal BsaI site found at 8378
    Illegal BsaI.rc site found at 6308
    Illegal BsaI.rc site found at 8367
    Illegal BsaI.rc site found at 9099
    Illegal BsaI.rc site found at 11477
    Illegal SapI.rc site found at 12576
    Illegal SapI.rc site found at 13326
    Illegal SapI.rc site found at 14553
    Illegal SapI.rc site found at 15303


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Categories
Parameters
None