Plasmid_Backbone

Part:BBa_K5293017

Designed by: Arnaud Boudigou   Group: iGEM24_uOttawa   (2024-09-23)
Revision as of 12:33, 30 September 2024 by Arnaud-boudigou (Talk | contribs)


pHREAC_eGFP_APO

pHREAC plasmid backbone for apoplasm targeted protein expression. Sap I sites are used to clone you insert (GGC & TGA are the scars). This empty plasmid backbone contains an insert not displayed on the sequence for easier cloning with mCherry as a screening tool.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2326
    Illegal EcoRI site found at 4024
    Illegal XbaI site found at 922
    Illegal SpeI site found at 5870
    Illegal PstI site found at 8527
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2326
    Illegal EcoRI site found at 4024
    Illegal NheI site found at 3235
    Illegal SpeI site found at 5870
    Illegal PstI site found at 8527
    Illegal NotI site found at 5724
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2326
    Illegal EcoRI site found at 4024
    Illegal BglII site found at 1289
    Illegal BglII site found at 2552
    Illegal BglII site found at 2572
    Illegal BglII site found at 9342
    Illegal BamHI site found at 928
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2326
    Illegal EcoRI site found at 4024
    Illegal XbaI site found at 922
    Illegal SpeI site found at 5870
    Illegal PstI site found at 8527
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2326
    Illegal EcoRI site found at 4024
    Illegal XbaI site found at 922
    Illegal SpeI site found at 5870
    Illegal PstI site found at 8527
    Illegal NgoMIV site found at 3036
    Illegal NgoMIV site found at 4194
    Illegal NgoMIV site found at 4720
    Illegal NgoMIV site found at 5596
    Illegal NgoMIV site found at 5720
    Illegal NgoMIV site found at 6747
    Illegal NgoMIV site found at 7338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


[edit]
Categories
Parameters
None