Composite

Part:BBa_K5097008

Designed by: Kelly Acen, Marie Box, Alexia Darby, Jena Dookie, Blake Dieckman, Runya Chaora, Chris Dorce, Adrianna Dugan, Elber Lopez-Hernandez, kayla VanPelt, Makenna Ventuleth   Group: iGEM24_Oneonta   (2024-09-28)
Revision as of 12:25, 30 September 2024 by Elberlopezh (Talk | contribs)


pASR-RFP reporter

Usage and Biology

The 2024 Oneonta iGEM team pHish and CHIPs created this acid-sensitive reporter circuit to characterize the expression of genes in response to acidic conditions, using pASR (BBa_K1231000). To do this, we used pARS to drive the expression of RFP, a reporter gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 707
    Illegal AgeI site found at 819
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None