Coding

Part:BBa_K5301008

Designed by: Jiaxin Guan   Group: iGEM24_BNU-China   (2024-09-22)
Revision as of 10:33, 30 September 2024 by Guanjiaxin (Talk | contribs)

MCherry [1-10] is a part of bimolecular fluorescence complementation (BiFC) system.

MCherry [1-10] is a part of bimolecular fluorescence complementation (BiFC) system which could visualize protein interactions. The combination of mCherry [1-10] and mCherry [11] can form the fluorescence of mCherry. With its advantages of a short maturation time and brilliant fluorescence, the mCherry BiFC system could find particular applications for analyzing protein–protein interactions especially in living cells [1].

Usage and Biology

we do sth.

Characterization

We used SDS-PAGE to test whether

sds-page-of-spnw50-1-test.png

Fig.1


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 31
    Illegal PstI site found at 230
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 31
    Illegal PstI site found at 230
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 31
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 31
    Illegal PstI site found at 230
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 31
    Illegal PstI site found at 230
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ Fan, J., et al., Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein–protein interactions in living cells. Biochemical and Biophysical Research Communications, 2008. 367(1): p. 47-53.
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