Part:BBa_K5207010

pTDH3-SmGGPPS-tENO1

This is an expression cassette consisting of a promoter pTDH3, the SmGGPPS gene, and a terminator tENO1.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 685
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 861
  
• 1000
  COMPATIBLE WITH RFC[1000]

Usage and Biology

We combined each Level 0 junction module, promoter, CDS, terminator, and ending module, and constructed them into vector pYTK095 through BsaI digestion and ligation, and this step was the construction of Level 1.

Figure 1. Snapgene diagrams of pYTK095

After transformation in E. coli DH10B, we selected the single bacterial colony (pYTK095) that does not fluoresce under UV light from the Petri dishes. It was used for colony PCR amplification and validation.

Figure 2. Fluorescent labeling screening of pYTK095

Figure 3. Validation plot of pYTK095 gel electrophoresis