Coding

Part:BBa_K5235000

Designed by: Rui Fan   Group: iGEM24_SHSBNU-China   (2024-09-30)
Revision as of 09:00, 30 September 2024 by A13716990344 (Talk | contribs)


BsAld

This part contains two enzymes linked together. First is BsAld, from Bacillus subtilis, is the first step in theanine synthesis, converting pyruvate to alanine. BsAld first binds with NADH to form an enzyme-cofactor complex. Then, pyruvate binds to the active site of the enzyme-cofactor complex, and NADH transfers its electrons to the pyruvate, reducing the carbonyl group (C=O) to an imine (C=NH). The imine intermediate reacts with ammonium ions to form a Schiff base intermediate, which is then hydrolyzed to produce alanine, releasing NAD⁺ and water.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None