Part:BBa_K5235000
BsAld
This part contains two enzymes linked together. First is BsAld, from Bacillus subtilis, is the first step in theanine synthesis, converting pyruvate to alanine. BsAld first binds with NADH to form an enzyme-cofactor complex. Then, pyruvate binds to the active site of the enzyme-cofactor complex, and NADH transfers its electrons to the pyruvate, reducing the carbonyl group (C=O) to an imine (C=NH). The imine intermediate reacts with ammonium ions to form a Schiff base intermediate, which is then hydrolyzed to produce alanine, releasing NAD⁺ and water.
CsAlaDC, from *Camellia sinensis*, is the second step in theanine synthesis, converting alanine to ethylamine. CsAlaDC requires the cofactor pyridoxal phosphate (PLP), which is the active form of vitamin B6. Initially, the amino group of alanine forms a Schiff base intermediate with the aldehyde group of PLP. In the Schiff base intermediate, electrons on the α-carboxyl group are redistributed, subsequently removing α-hydrogen as carbon dioxide, forming a stable resonance structure. The resulting carbanion accepts a proton, forming the PLP-bound form of ethylamine. Ethylamine dissociates from PLP, restoring PLP to its original state, and the enzyme can proceed to the next catalytic cycle.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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