Coding

Part:BBa_K5271005:Design

Designed by: Yin Yan Chan   Group: iGEM24_HKPOLYU   (2024-09-29)
Revision as of 08:05, 30 September 2024 by Carriechan1 (Talk | contribs) (→‎Design Notes)


Fc fragment


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 165
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 342
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Avoid fusing Fc fragment to nanobody when using a prokaryotic system. Insufficient secretion and formation Inclusion bodies were found when expressing the Fc-fused nanobody in E. Coli.

Source

Fc Fragment

References