Composite

Part:BBa_K5036037

Designed by: Emad hamdy Matter   Group: iGEM24_AFCM-Egypt   (2024-09-21)
Revision as of 08:02, 30 September 2024 by Alisalah (Talk | contribs)


U6 promoter- Nanog gRNA3 (scaffold- spacer RNA )

Part Description

This part consists of the U6 promoter, which constantly activates the creation of our Nanog guide RNA, and The Nanog guide RNA comprises a spacer sequence that matches the Nanog DNA sequence, enabling gene editing. Additionally, the guide RNA includes a scaffold sequence that binds to the dCas9 protein, allowing it to interact with the target DNA

Usage

Our guide RNA is used with NLS to direct dCas9 linked to transcription activators VP64 and GAL4 into the nucleus to target Nanog gene which is located upstream to YAP-1 coding region which induce transcription and increase production of endogenous YAP-1.

This figure illustrates the function of Nanog gRNA in our model.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None